Ms. Tarantula is one of the three tritagonists (alongside Mr. Piranha and Mr. Shark) of the DreamWorks animated movie, The Bad Guys. She is a red-knee tarantula who is an expert hacker and the only female of the Bad Guys.
Obviously, don't nominate any human characters. Hybrids like Cheetah or The Lizard are okay, as are shapeshifters with one set animal form (eg Professor McGonagall.)
