This time we see the Mario brothers on vacation to Italy. But at their trip Mario discovers a hidden catacombs along a stretch of beach. Inside these catacombs, the brothers and Daisy are thrusted into a world within worlds and the evil King Boo is its ruler. Once there, Mario and Daisy are taken captive. In order for Luigi to save their lives, he must battle villains around every corner and for every level he defeats, the challenge becomes more diificult. Along the way, Luigi meets the genius, Professor Gadd and the beautiful fallen princess, Peach. In their combined forces, the brothers and their new friends must defeat Tatanga, Birdo, Boo...and an old adversary.
