The heroes Mario and Luigi goes to a magic college called Magical Leaf University after many adventures to take a big break, however, one day, when danger rises yet again! But this time the evil Mr. Dark, who is returned, decides to invade the school, Mario, Luigi, along with new friends, Rayman, Globox, SpongeBob and Patrick, protect the school from his foolproof plans, using their old powers, while finding new powers, discovering their pasts and befriending new students.
