Peter Parker, Gwen Stacy, Harry Osborn, and Mary Jane Watson remaining as teenagers in high school during the majority of the series, the introduction of Kenny McFarlane into animated medium, Eddie Brock's personal relationship with Peter while working as a lab assistant for Dr. Curt Connors at Empire State University, Rhino's real name being Alex O'Hirn, Dr. Octavius working as an Oscorp scientist until his electromagnetic accident.
Charlie Heaton as Spider-Man.
