The film is set in the Old West, and centers on a mismatched trio of dairy cows—brash, adventurous Maggie; prim, proper Mrs. Calloway; and ditzy, happy-go-lucky Grace. The three cows must capture an infamous cattle toed Alameda Slim for his bounty in order to save their idyllic farm from foreclosure. Aiding them in their quest is Lucky Jack, a feisty, peg-legged rabbit, and a selfish horse named Buck, eagerly working in the service of Rico, a famous bounty hunter, who seeks the glory for himself.