2 years after Mr. Freeze's defeat, Batman (Keaton), Batgirl (Silverstone), and Robin (O'Donnell) face a mentally unstable scientist named Jonathan Crane (Englund), also known as "The Scarecrow" and Harley Quinn (Madonna), a toymaker and daughter of the Joker bent on revenge against Batman for her father's death. Crane offers an alliance with Quinn to end his The Batman for good.