The player begins experiencing the life of Aveline in 1765, as she operates within the city of New Orleans from her family mansion, which she lives in alongside her stepmother, Madeleine de L'Isle. As the city undergoes a transition of control from France to Spain near the end of the French and Indian War, Aveline discovers a Templar plot to control Louisiana during the transitional period being conducted by the Templar Rafael Joaquín de Ferrer. To counter this, she assassinates two men assisting in his goals - French governor Jean-Jacques Blaise d'Abbadie, as part of a deal to maintain his post in New Orleans, and Baptise, a defector from the Assassins who attempts to control the smuggling operations in the Bayou around the city and lure Aveline's mentor, the Assassin Agaté, out of hiding. A year after these assassinations, de Ferrer is sent to a Templar worksite in Mexico, while his associate Antonio de Ulloa oversees New Orleans as its new Spanish governor, imposing strict trading restrictions, setting up a covert slave-trading operation, and allowing French officials to continue operating in the city.
